Nanopore immunosensor for peanut protein Ara h1
نویسندگان
چکیده
A nanopore immunosensor is demonstrated for peanut protein Ara h1, a common food allergen. This biosensor is constructed by immobilizing the antibody to peanut protein Ara h1 within Au-coated pores of commercial nanoporous polycarbonate membranes. Peanut protein Ara h1 is detected as the change in the pore conductivity as the pores are partially obscured by antigen binding. Control experimentswith the antibody to cockroach protein Bla g1 demonstrate that the observed effects do not arise from nonspecific adsorption. Binding of peanut protein is studied as a function of the pore diameter (15, 30 and eywords:
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